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OBJECTIVE： To screen active fractions of Xiaozhong zhitong lotion that are able to reduce swelling， promote ulcer healing and analgesia， and to provide reference for it’s secondary development of ointment preparation. METHODS： Water elution fraction and 20％， 40％， 60％， 95％ ethanol elution parts were separated by D101 macroporous resin from Xiaozhong zhitong lotion. 120 SD rats were randomly divided into normal group （n＝8） and modeling group （n＝112）. Rats in the normal group were not treated. Hemorrhoids model was established in the model group by injecting 75％ glacial acetic acid into the perianal skin to induce perianal ulcer. 96 model rats were randomly divided into model group [blank ointment matrix 0.51 g/（kg·d）]， positive group [Mayinglong ointment， 0.51 g/（kg·d）]， high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s water elution fraction and 20％， 40％， 60％ ethanol elution parts [8.34， 2.78 g/（kg·d） by crude drug， all make into containing drug ointment]， with 8 rats in each group. The periphery of the anus was smeared with relevant medicine， twice a day， for consecutive 7 d. The local symptoms around the anus of rats 3 and 7 days after administration and the pathological morphology of the local mucosa around the anus of rats 7 days after administration were observed and scored respectively. The effects of each elution part for reducing swelling and promoting ulcer healing were investigated. 120 ICR mice were randomly divided into model group [blank ointment matrix 1.03 g/（kg·d）]， positive group [Mayinglong ointment 1.03 g/（kg·d）]， high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s each elution part [16.65， 5.55 g/（kg·d） by crude drug， all make into containing drug ointment]， with 10 mice in each group. Transdermal administration， twice a day， for consecutive 7 d. After 30 min of last administration， the latency time and 15 min writhing times of mice were detected by designing acetic acid writhing test； pain threshold of mice was determined by hot-plate pain test so as to investigate systemic and local analgesic effects of each elution part. RESULTS： In the detumescence and ulcer healing test， compared with normal group， the score of local symptoms around anus at 3rd and 7th day of administration as well as pathological score of hemorrhoids local mucosa were increased significantly in model group （P＜0.01）. Compared with model group， above scores of positive group， 40％ ethanol elution high-dose and low-dose groups were decreased significantly （P＜0.05 or P＜0.01）. The scores of local symptoms around anus in 20％ ethanol elution part high-dose group at 3rd and 7th day after medication as well as 60％ ethanol elution part group at 7th day after medication were decreased significantly （P＜0.05）. In analgesia test， compared with model group， writhing latency time was shortened significantly and 15 min writhing times was decreased significantly in positive group， 40％ ethanol elution part high-dose and low-dose groups as weel as 60％ ethanol elution part high-dose group （P＜0.05 or P＜0.01）； writhing latency time of 20％ ethanol elution part high-dose group was shortened significantly （P＜0.05）. Pain threshold of mice was increased significantly in positive group， 40％ ethanol elution part high-dose and low-dose groups after medication （P＜0.05 or P＜0.01）. CONCLUSIONS： The 40％ ethanol elution part from Xiaozhong zhitong lotion is the effective part that can reduce swelling， promote ulcer healing and analgesia.
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OBJECTIVE： To screen the active part of Saccharum officinarum leaves against liver injury. METHODS： The S. officinarum leaves were extracted with 80％ ethanol to obtain ethanol extract， after dispersed with water， ethanol extract was respectively extracted with petroleum ether， ethyl acetate and n-butanol to obtain corresponding polar parts. The residual part was water part. Totally 108 mice were randomly divided into blank group A （0.5％ CMC-Na and 3.5％ polysorbate 80 solution）， blank group B （purified water）， model group （purified water）， biphenyl diester group （positive control， 0.2 g/kg）， S. officinarum leaves ethanol extract group， different polar parts of S. officinarum leaves group （petroleum ether， ethyl acetate， n-butanol， water， 22.92 g/kg crude drug， 0.5％ CMC-Na and 3.5％ polysorbate 80 solution as solvent）， with 12 mice in each group. They were given relevant medicine intragastrically once a day， for consecutive 12 d. 1 h after last medication， except for blank group A and blank group B， mice in other groups were given 0.15％ CCl4 peanut oil （0.1 mL/10 g） solution to induce acute liver injury model. 16 h later， general information of mice was observed， and the serum contents of ALT and AST were determined. The liver histopathological changes were observed and the Ishak scores were scored. RESULTS： Compared with blank group B， each index of blank group A had no significant difference （P＞0.05）. Compared with blank group A， model group had sparse hair and slow movement， and was emaciated. Serum contents of ALT and AST were increased significantly （P＜0.01）. The structure of hepatic lobule was severely damaged； the structure of hepatic cord and sinus was not clear； the arrangement of hepatic cord was disordered， and the Ishak score was significantly increased （P＜0.01）. Compared with model group， general information of mice was improved in administration groups. Serum contents of ALT and AST were decreased in biphenyl diester group， S. officinarum leaf ethanol extract group and S. officinarum leaf ethyl acetate group （P＜0.05 or P＜0.01）. The pathological damage of liver tissue was significantly relieved， and Ishak score was significantly reduced （P＜0.05 or P＜0.01）. CONCLUSIONS： The ethyl acetate part of ethanol extract from S. officinarum leaves is active part against CCl4-induced acute liver injury of mice.
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Objective:To further confirm the anti-endotoxin active parts of Lidanpaidu prescription through observing the anti-en-dotoxin intensity based on a half-in vivo method. Methods:The extracting solution of Lidanpaidu prescription was extracted by the sol-vents with different polarity to obtain the active part. The anti-endotoxin activity of the samples was determined by using the Azo color matrix method, and the amount of inactivated endotoxin was compared among the various extraction parts with different dosages. Re-sults:Compared with that of the control group, the amount of inactivated endotoxin by the rat plasma in each administration group was all higher, and the differences were statistically significant (P0. 05). The rat plasma of the water soluble fraction administration groups also showed endotoxin inactivation, and the difference be-tween the high dosage group and the low dosage group was statistically significant (P<0. 05). Conclusion:The results of the half-in vivo method showed the consistent anti-endotoxin effect of the whole prescription and the water soluble fraction, furthermore, the dose-effect relationship is preliminarily appeared, suggesting that the water soluble fraction remains the effective components of the whole prescription to a great extent. Therefore, the water soluble fraction can be further confirmed as the active anti-endotoxin part of Lidan-paidu prescription.
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OBJECTIVE:To compare the pharmacodynamics of active parts in crude Atractylodes lancea and A. lancea fired with bran. METHODS:170 rats were randomly divided into 17 groups,including blank control group,spleen and stomach damp obstruction model group,volatile oil of crude A. lancea and A. lancea fired with bran high-dose and low-dose(0.747,0.083 mg/ml by the concentration of crude medicinal materials,similarly hereinafter)groups,solvent control 2% polysorbate 80 group,dichlo-romethane extract of crude A. lancea and A. lancea fired with bran high-dose and low-dose groups,solvent control 1‰ polysorbate 80 group,n-butyl alcohol extract of crude A. lancea and A. lancea fired with bran high-dose and low-dose groups,solvent control stomach damp obstruction model distilled water control group. Except blank control group,other 16 groups were given Sennae foli-um decoction ig for 14 d to induce spleen and stomach damp obstruction model,and then received relevant medicine or solvent ig once a day for consecutive 7 d. Body weight of rats were determined before and after medication,and the serum levels of amylase, D-xylose,gastrin,vasoactive intestinal peptide and NO were determined after medication. RESULTS:Compared with spleen and stomach damp obstruction model group,the body weight and serum levels of gastrin,amylase and D-xylose increased significantly in rats of active part in A. lancea fired with bran groups(P<0.05),while vasoactive intestinal peptide and NO decreased signifi-cantly(P<0.05). Compared active part in crude A. lancea group,except the body weight,above indicators of active part in A. lan-cea fired with bran groups had greater change,with statistical significance (P<0.05). CONCLUSIONS:There are pharmacody-namic differences in the active parts between crude A. lancea and A. lancea fired with bran,the latter one is stronger.
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[Objective] To observe effects of HQSM on CK, LDH and AST activity in serum of acute myocardial ischemia rats caused by injecting pituitrin.[Method] SD rats were divided into several groups at random: normal control group,model control group, three HQSM groups(210mg/kg,150mg/kg,105mg/kg), positive control group(Huangqi Shengmaiyin and Diao Xinxuekang).The rats were treated with respective drugs by ig for 30 days, and pituitrin was injected respectively by caudal vein 1h after administering at the 29th and 30th days.Blood serum was taken out to determinate the activity of CK, LDH and AST at 1h and 24h after the last injection. [Result] The activity of LDH and AST in serum increased at 1h after injecting pituitrin, the activity of CK in serum increased,meanwhile LDH and AST was still higher than normal control group at 24h. HQSM could decrease the activity of CK and AST(P
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OBJECTIVE:To observe the superiority of countercurrent chromatography(CCC)in isolating active componets of botanicals.METHODS:By pharmacodynamic test,active components of herbs,isolated by CCC,were screened.RESULTS:An active part of herb was found and a component with a purity of95%was obtained.CONCLUSION:CCC is a rapid,effective and unabsorpting isolation technology compared with other methods in developing herbal researches.